[바이오세상] Western Blot 주요 제품
2022-03-29
: Cell 또는 조직에서 Protein 추출 후 Sample 준비
| Product Name | Cat No. | Standard |
| (R2002) RIPA Buffer | RC2002-050-00 | 500 ml |
| (M1015) 2-MERCAPTOETHANOL BIOTECHNOLOGY GRADE(RT)(C2H6OS) | MR1015-100-00 | 100 ml |
| (D1037) DTT(C)(C4H10O2S2) | DC1037-005-02 | 5 g |
| (B1017) BRADFORD KIT | BC1017-500-02 | 500 ml |
| (B2005) BSA Solution (20mg/mL) | BF2005-001-00 | 1 mL |
: SDS로 Protein을 (-) charge로 띄게 한 후 (+) charge를 연결해 Protein 분자량 별로 분리
| Product Name | Cat No. | Standard |
| (S2003) 10% SDS SOLUTION | SR2003-050-00 | 500 ml |
| (A2013) 10% Ammonium Persulfate Solution (냉장) | AC2013-110-00 | 10 ml |
| (A2003-1) 30% Acrylamide-Bis Solution(29:1)(유독)-10 | AC4003-050-00 | 500 ml |
| (A2004-1) 30% Acrylamide-Bis Solution (37.5:1)(유독)-10 | AC4004-050-00 | 500 ml |
| (T1004) TEMED(RT)(C6H16N2) | TR1004-025-00 | 25 ml |
| (T2020-1) 1.5M TRIS-HCl, pH 8.8 | TR4020-050-88 | 500 ml |
| (T2020-6.8) 1.5M TRIS-HCl, pH 6.8 | TR2020-050-68 | 500 ml |
| (S2002) 5X SDS-PAGE Loading Buffer | SF2002-110-00 | 10 ml |
| (T2015) 10X Tris-Glycine Buffer(SDS)-Running Buffer(일반조성) | TR2015-100-00 | 1 L |
: Gel 내부의 단백질은 항원이 결합할 수 없기 때문에 항원이 결합할 수 있는 Membrane으로 Size 별로 분리된 단백질을 이동
| Product Name | Cat No. | Standard |
| (T2028) 10X TRIS-GLYCINE BUFFER (W/O SDS) – Transfer Buffer | TR2028-100-00 | 1 L |
| (M1066) Methanol(RT)(CH3OH)(유독,사고대비)-5 | MR1066-500-01 | 500 ml |
| (C2005) Coomassie Brilliant Blue G-250 Staining | CR2005-100-00 | 1 L |
| (C2006) Coomassie Brilliant Blue R-250 (상온) | CR2006-100-00 | 1 L |
| (P2059) 0.2% Ponceau S solution | PR2059-050-00 | 500 ml |
| (D2018) Destaining Solution | DR2018-100-00 | 1 L |
: 단백질이 Transfer 되지 않은 부분이 다른 단백질로 오염되거나 Antibody가 붙을 수 없도록 Skim Milk 나 BSA 등으로 Blocking
|
Product Name |
Cat No. | Standard |
| (T2007) 10X TBS WITH TWEEN-20 | TR2007-100-74 | 1 L |
| (T2005) 10X TBS, pH 7.4 | TR2005-100-74 | 1 L |
| (P2007-1) 10X PBS | PR4007-100-00 | 1 L |
| (A1025) ALBUMIN BOVINE, FRACTION V (BSA) | AC1025-005-00 | 50 g |
: 2nd Antibody의 HRP가 ECL의 luminol을 산화시켜 방출되는 빛을 Detection
|
Product Name |
Cat No. | Standard |
| (W6002) ECL plus kit | WC6002-200-KT | 200 ml |
| (S2039) Stripping Buffer | SR2039-100-00 | 1 L |
* Protein PAGE Solution
Protein PAGE Solution + TEMED(TR1004-025-00) + 10% APS Solution = Acrylamide gel solution
|
Product Name |
Cat No. | Standard |
| (P2016) PROTEIN-PAGE-5%(29:1) Stacking Gel | PC2016-025-00 | 250 mL |
| (P2018) Protein-PAGE-6% (29:1) Resolving Gel | PC2018-025-00 | |
| (P2019) Protein-page-6% (37.5:1) Resolving gel | PC2019-025-00 | |
| (P2020) PROTEIN-PAGE-8% (29:1) Resolving Gel | PC2020-025-00 | |
| (P2021) Protein-PAGE-8% (37.5:1) Resolving Gel | PC2021-025-00 | |
| (P2022) Protein-PAGE-10% (29:1) Resolving gel | PC2022-025-00 | |
| (P2023) Protein-PAGE-10% (37.5:1) Resolving Gel | PC2023-025-00 | |
| (P2024) Protein-PAGE-12% (29:1) Resolving gel | PC2024-025-00 | |
| (P2025) Protein-PAGE-12% (37.5:1) Resolving Gel | PC2025-025-00 |
* Western Blot 실험 방법에 관한 상세한 설명은 아래 링크 참고 부탁 드립니다.
