[Cell Signaling Technology] Western Blot Controls
2023-03-24
· Loading Control
– Loading Control은 동일한 양의 Protein Sample이 Loading 되었는지 정량적인 비교를 위해 사용
– House-keeping Gene이나 모든 Cell/Tissue에서 동일하게 발현하는 Protein을 Loading Control로써 사용
– Loading Control은 필수적
| Loading Control | Size (kDa) | Recommended for: |
| β-Actin | 45 | Whole Cell Lysate |
| GAPDH | 37 | |
| β-Tubulin | 55 | |
| α-Tubulin | 52 | |
| Vinculin | 124 | |
| α-Actinin | 100 | |
| Cyclophilin B | 19 | |
| Loading Control | Size (kDa) | Recommended for: |
| Histone H3 | 17 | Nuclear Fraction |
| HDAC1 | 62 | |
| Loading Control | Size (kDa) | Recommended for: |
| COX IV | 17 | Mitochondrial Fraction |
| VDAC | 32 | |
| SDHA | 70 |
· Positive Control
– Positive Control Lysate는 Target Protein을 발현한다고 알려진 Cell Line이나 Tissue에서 채취한 Sample
– Target Protein이 Antibody로 인해 정확하게 Detection이 되었는지, 실험 Protocol에는 이상이 없었는지 확인하는 목적
– 실험 Sample과 Positive Control을 비교하여 Sample의 발현량 확인 가능
· Negative Control
– Negative Control Lysate는 Target Protein을 발현하지 않는다고 알려진 Cell Line이나 Tissue에서 채취한 Sample
– Non-specific Binding 유무 확인
| Cat No. | Product name |
| 41645S | NRF2 Control Cell Extracts |
| 94790S | HIF-1α/2α Control Cell Extracts |
| 33263S | CHOP Control Cell Extracts |
| 51367S | 4E-BP1 Control Cell Extracts |
| 34499S | p70 S6 Kinase MCF7 Control Cell Extracts |
| 11972S | LC3 Control Cell Extracts |
| 12052S | Smad2/3 Control Cell Extracts |
| 5634S | EGF Receptor Control Cell Extracts |
| 9194S | p44/42 MAPK (Erk1/2) Control Cell Extracts |
| 9158S | AMPK Control Cell Extracts |
| 9160S | MEK1/2 Control Cell Extracts |
| 2043S | Jurkat Apoptosis Cell Extracts (etoposide) |
| 9663S | Caspase-3 Control Cell Extracts |
| 9253S | SAPK/JNK Control Cell Extracts |
| 9193S | CREB Control Cell Extracts |
| 9133S | Stat1/2/3/5 Control Cell Extracts |
| 9213S | p38 MAPK Control Cell Extracts |
| 9233S | MKK3/MKK6 Control Cell Extracts |
| 9223S | ATF-2 Control Cell Extracts |
| 9273S | Akt Control Cell Extracts |
| 9263S | c-Jun Control Cell Extracts |
