[Cell Signaling Technology] CST FastScan&PathScan ELISA Kit

 

 

Sandwich ELISA Assay

 

– 항원(antigen)에 대한 항체(antibody)를 먼저 Plate에 결합시키고, 그 항체(antibody)에 항원(antigen)을 결합시킨 다음 직접법이나 간접법으로 조사하는 방법

– 항원(antigen)의 양이 적을 때 또는 미지의 샘플에 들어 있는 항원(antigen)을 조사할 때 사용

 

a) 항원 (antigen) 의 양을 측정

b) 두 개의 항원-특이적 항체가 요구됨 (Antibody pairs)

c) 높은 민감도

 

 

 

FastScan™ ELISA	PathScan® ELISA

 

 

Assay type	Solution-based sandwich	Solid-phase sandwich
Hands-on Time	15 min	Kits (pre-coated plates): 25 min Pairs (plates coated by user): >45 min
Number of Wash	1 time	Many times
Blocking	Unnecessary	Necessary
Typical Time to Result	90 mins	Kits (pre-coated plates): 240 min Pairs: (plates coated by user): >240 min – Overnight
Sensitivity	Higer Sensitivity than Traditional ELISA	Comparable to Traditional ELISA
Dynamic Range	Comparable to Traditional ELISA
Sample Types	Cell Extract, Tissue Extract, Other
Internal Control	Yes	No
Quantitation	Semi-quantitative
Detection	Colorimetric	Colorimetric – All Chemiluminescence – Partial
Types of Targets	Post-translational Modifications, Endogenous Cellular Proteins