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[Cell Signaling Technology] CST FastScan&PathScan ELISA Kit

2023-03-10

 

 

Sandwich ELISA Assay

 

– 항원(antigen)에 대한 항체(antibody)를 먼저 Plate에 결합시키고, 그 항체(antibody)에 항원(antigen)을 결합시킨 다음 직접법이나 간접법으로 조사하는 방법

– 항원(antigen)의 양이 적을 때 또는 미지의 샘플에 들어 있는 항원(antigen)을 조사할 때 사용

 

a) 항원 (antigen) 의 양을 측정

b) 두 개의 항원-특이적 항체가 요구됨 (Antibody pairs)

c) 높은 민감도

 

 

 

FastScan ELISA PathScan® ELISA

 

 

Assay type Solution-based sandwich Solid-phase sandwich
Hands-on Time 15 min Kits (pre-coated plates): 25 min
Pairs (plates coated by user): >45 min
Number of Wash 1 time Many times
Blocking Unnecessary Necessary
Typical Time to Result 90 mins Kits (pre-coated plates): 240 min
Pairs: (plates coated by user): >240 min – Overnight
Sensitivity Higer Sensitivity than Traditional ELISA Comparable to Traditional ELISA
Dynamic Range Comparable to Traditional ELISA
Sample Types Cell Extract, Tissue Extract, Other
Internal Control Yes No
Quantitation Semi-quantitative
Detection Colorimetric Colorimetric – All
Chemiluminescence – Partial
Types of Targets Post-translational Modifications, Endogenous Cellular Proteins