BD Intracellular Flow Cytometry

2021-07-02

기본원리

타겟 단백질의 세포 내 위치에 따라 적합한 Fixation/Permeabilization buffer를 처리한 후, 타겟 단백질에 대한 형광 항체를 염색하여 분석합니다.

Detection Cytokine

Transport inhibitor를 처리하여 사이토카인(Cytokine)/케모카인(Chemokine)의 분비를 막은 후 분석합니다.

품명	구성품	용량	품번
BD Cytofix/Cytoperm™ Solution Kit	Perm/Wash Buffer Fixation/Permeabilization Solution	250 Tests	554714
BD Cytofix/Cytoperm™ Solution Kit with BD GolgiStop™	Perm/Wash Buffer Fixation/Permeabilization Solution BD GolgiStop™ Protein Transport Inhibitor®	250 Tests	554715
BD Cytofix/Cytoperm™ Solution Kit with BD GolgiPlug™		250 Tests	555028
Perm/Wash Buffer		100 mL	554723
Fixation/Permeabilization Solution		125 mL	554722
BD GolgiStop™ Protein Transport Inhibitor	Monensin solution	0.7 mL	554724
BD GolgiPlug™ Protein Transport Inhibitor	Brefeldin A solution	1 mL	555029
BD IntraSure™ Kit	Reagent A (Fix) Reagent B (Perm)	50 Tests	641776

추천 Transport Inhibitor

Species	Cytokines	Transport Inhibitor
Human	IL-1α, IL-6, IL-8, TNF-α	Monensin
Human	IFN-γ, IL-2, IL-10, IL-12, MCP-1, MCP-3, MIG, MIP-1α, RANTES	Either monensin or brefeldin A
Mouse	IL-6, IL-12, TNF-α	Brefeldin A
	GM-CSF, IL-3, IL-4, IL-5, IL-10	Monensin
	IFN-γ, IL-2	Either monensin or brefeldin A

Fastimmune

Whole Blood Assay로 빠른 분석이 가능합니다.

품명	용량	품번
Brefeldin A Solution	250 ul	347688
BD FACS Permeabilizing Solution 2	200 Tests	347692
BD FACS Permeabilizing Solution 2	500 Tests	340973
Fastimmune EDTA Solution	2.5 mL	347689
BD Lysing Solution	100 mL	349202

Detection of Transcription Factors

FoxP3, Sox17과 같은 Transcription factor의 분석을 위한 버퍼

품명	구성품	용량	품번
Transcription Factor Buffer Set	TF Fix/Perm Buffer TF Diluent Buffer TF Perm/wash Buffer	25 Tests	562725
Transcription Factor Buffer Set	TF Fix/Perm Buffer TF Diluent Buffer TF Perm/wash Buffer	100 Tests	562574
Human FoxP3 Buffer Set	Human FoxP3 Buffer A Human FoxP3 Buffer B	100 Tests	560098
Mouse Foxp3 Buffer Set	Mouse Foxp3 Fixation Concentrate Mouse Foxp3 Permeabilization Concentrate	100 Tests	560409

Detection of Phosphorylated Protein

단일 세포(Single cell)가 가진 타겟 단백질의 발현 수준을 분석할 수 있으며, 타겟 단백질의 epitope 위치에 따라 버퍼를 구분하여 사용합니다.

품명	설명	용량	품번
BD Phosflow™ Lyse/Fix Buffer		250 mL	558049
BD Phosflow™ Fix Buffer I		250 mL	557870
BD Phosflow™ Perm/Wash Buffer I	Mild Detergent Method	125 mL	557885
BD Phosflow™ Perm Buffer II	Mild Alcohol Method (64.86%)	125 mL	558052
BD Phosflow™ Perm Buffer III	Harsh Alcohol Method (87.68%)	125 mL	558050
BD Phosflow™ Perm Buffer IV	Harsh Detergent Method	50 mL	560746

기타 Buffer

품명	설명	용량	품번
BD Pharm Lyse™ Lysing Buffer (10X concentrate)	Ammonium Chloride-based Lysing Reagent	100 mL	555899
BD Cytofix™ Fixation Buffer		100 mL	554655
BD Cytoperm™ Permeabilization Buffer Plus	BrdU Flow Kit용	10 mL	561651

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