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세포 내 사이토카인 분석을 위한 BD Biosciences 제품 알아보기

2025-09-19

 

Intracellular Flow Cytometry는 다양한 Cell type의 Cytokine / Chemokine production을 분석할 수 있습니다.

BD의 관련 제품들을 서린바이오사이언스에서 만나 보세요.

기본원리

Cell의 Fix/Perm 과정을 거쳐 세포 내/외부의 Target Protein 동시에 인지

* 이 때 Secretion 되는 Protein은 Inhibitor를 처리하여 세포 외부로의 분비를 방지

Detection of Cytokines

       Antigen-specific IFN-γ production by cytomegalovirus (CMV) pp65- stimulated CD4+CD69+ T lymphocytes.

[관련제품]

Description Contents Size Cat.
BD Cytofix/Cytoperm™ Solution Kit Perm/Wash Buffer

Fixation/Permeabilization Solution

 250 Tests 554714
BD Cytofix/Cytoperm™ W/Golgi Stop Kit Perm/Wash Buffer

Fixation/Permeabilization Solution

BD GolgiStop™ Protein Transport Inhibitor*

 250 Tests 554715
BD Cytofix/Cytoperm™ W/GolgiPlug Kit Perm/Wash Buffer

Fixation/Permeabilization Solution

BD GolgiPlug™ Protein Transport Inhibitor*

 250 Tests 555028
Fixation/Permeabilization Solution 125 ml 554722
Perm/Wash Buffer 100 ml 551423
BD GolgiStop™ Protein Transport Inhibitor* Monensin solution 0.7 ml 554724
BD GolgiPlug™ Protein Transport Inhibitor* Brefeldin A solution 1 ml 555029
BD IntraSure™ Kit Reagent A (Fix) / Reagent B (Perm) 50 Tests 641776

**추천 Transport Inhibitor

Species Cytokine Transport Inhibitor
Human IL-1α, IL-6, IL-8, TNF-α Monensin
IFN-γ, IL-2, IL-10, IL-12, MCP-1, MCP-3, MIG, MIP-1α, RANTES Either monensin or brefeldin A
Mouse IL-6, IL-12, TNF-α Brefeldin A
GM-CSF, IL-3, IL-4, IL-5, IL-10 Monensin
IFN-γ, IL-2 Either monensin or brefeldin A

 

 

 

 

#BD #BDBiosciences #서린바이오사이언스 #서린바이오 #FlowCytometry #IntracellularFlowCytometry #유세포분석 #세포내염색
#Cytokine #Chemokine #BDCytofix #BDCytoperm #BDIntraSure #BDGolgiStop #BDGolgiPlug #Monensin #BrefeldinA #ProteinTransportInhibitor #Fixation #Permeabilization #FACSLyric #BD시약 #BD제품 #BD제품주문 #면역세포분석 

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