BD Biosciences T-Cell Research


BD Biosciences 대리점, 서린바이오사이언스 입니다.


T-Cell Subtypes        cytotoxic T cells / helper T cells(Th) / regulatory T cells(Tregs)

Type of Cell Cytotoxic Th1 Th2 Th95 Th17 Tfh6 Treg
Main Function Kill virus-infected cells Activate microbicidal

function of
Kill virus-infected cells infected macrophages, and help B cells
to produce antibody

Help B cells and switch antibody isotype production T cell proliferation and neutrophil response production by B cells Enhance neutrophil


Regulate development of antigen specienhanced IgG and IgE Enhance fic B cell development and antibody production Immune regulation
Extracellular Markers CD8 CD4 CXCR3 CD4 CCR4, Crth2 (human) CD4 CD4, CCR6 CD4, CXCR5 CD4, CD25


IFN-g, IL-2, IL-12, IL-18, IL-27 IL-4, IL-2, IL-33 IL-4,TGF-b TGF-b, IL-6, IL-1, IL-21, IL-23 IL-12, IL-6 TGFb- , IL-12
Effector Cytokines IFN-g,TNF, LT-a IFN-g, LT-a,TNF IL-4, IL-5, IL-6, IL-13 IL-9, IL-10 IL-17A, IL-17F, IL-21, IL-22, IL-26 ,TNF, CCL20 IL-21 TGF-b, IL-10
Transcription Factors T-bet,Stat1,Stat6 GATA3,Stat5,Stat6 GATA3,Smads,Stat6 RORgt, RORa, Stat3 Bcl-6, MAF FoxP3,Smad3,Stat5

– To support the use of multicolor flow cytometry for the study of T cells, BD offers a deep portfolio of reagents, which are highlighted in pink.


T-Cell Research Platforms

Cell Surface Markers

: To Identify cells from heterogeneous samples

[Phenotyping of Cells with Unique Surface Profiles]

T cells and their subsets can be identified by differential expression of cell surface markers including CD3, CD4, CD8, CD25, CD127, and CD196 (CCR6).

Adding markers such as CD197 (CCR7), CD62L, CD69, and CD45RO to an analysis provides important information about the potential for cells to home and

localize within the body, as well as the activation status of the T-cell subset of interest. This information can also be used to identify different memory subsets.

With the availability of multiple BD Horizon Brilliant Violet and Brilliant Ultraviolet dyes, larger panels can be created that include multiple dim markers.

Rich data sets can be obtained from precious samples. The 13-color panel below examines memory and activation status of multiple T-cell subsets.


Marker Fluorochrome Purpose
Viability dye V500 Viability
CD3 PerCP-Cy5.5 T cell marker
CD4 BUV395 T cell subsetting
CD8 FITC T cell subsetting
CD127 Alexa Fluor® 647 RegulatoryTCell Marker
HLA-DR APC-H7 Activation
CD45RO PE-Cy7 Memory
CD197 (CCR7) BV421 Naïve/Memory
CD38 BV605 Activation
CD27 BV786 Memory
CD25 PE-CF594 Regulatory T Cell


CD196 (CCR6) PE Th17 Cell Marker
CXCR3 Alexa Fluor® 700 Th1 Cell Marker
Intracellular flow cytometry

: A powerful tool for the study of T-cell differentiation

BD Cytofix/Cytoperm

: fixation/permeabilization solution

– 다양한 Cytokines 및 Cell surface marker

   staining 목적으로 사용

BD Pharmingen

: transcription factor buffer set

– Transcription factor alone or Cell surface

marker, cytokines과 혼합되어 있는

Transcription factor staining 목적으로 사용

 BD Phosflow

: permeabilization buffer III

– Phosphoepitope 검출을 위한 Flow cytometry

분석 시 Permeabilization 목적으로 사용

BD™ Cytometric Bead Array (CBA)

: To measure secreted cytokines within a sample


CBA products 장점

– 여러 cytokine을 동시에 분석 가능 (multiplexing)

– 소량의 샘플로도 Quantitative Result 획득 가능

– 타사 제품 대비 분석 소요 시간이 짧음


*CBA vs. ELISA vs. ICS 비교  *intracellular cytokine staining (ICS)

Allows detection of multiple cytokines in same experiment  ✓   ✓ 
Can obtain phenotype of specific cells expressing cytokine of interest  ✓ 
Can measure quantity of cytokine secreted  ✓   ✓