BD Biosciences 세포주기(Cell Cycle) 분석
2021-04-14
BrdU를 이용한 Cell Cycle 분석 원리
정확한 S기 분석을 위해서 BrdU (Bromodeoxyuridine)와 anti-BrdU 항체를 사용합니다.
DNA 합성과정에서 티미딘 (T, Thymidine)을 대신하여 BrdU가 새로 합성되는
DNA 가닥에 들어갈 수 있습니다.
Kit/Set
| 품명 | 구성품 | 용량 | 품번 |
| FITC BrdU Flow Kit | FITC labeled anti-BrdU antibody, 7-AAD, Permeabilization Buffer, Fixation and Permeabilization Solution, Perm/Wash Buffer, DNase, BrdU |
50 Tests | 559619 |
| 200 Tests | 557891 | ||
| APC BrdU Flow Kit | APC anti-BrdU, 7-AAD, Permeabilization Buffer, Fixation and Permeabilization Solution, Perm/Wash Buffer, DNase, BrdU | 50 Tests | 552598 |
| 200 Tests | 557892 | ||
| FITC Mouse Anti- BrdU Set | FITC Mouse Anti-BrdU, FITC Mouse IgG1 κIsotype Control | 100 Tests | 556028 |
| PE Mouse Anti- BrdU Set | PE Mouse Anti-BrdU, PE Mouse IgG1 κIsotype Control | 100 Tests | 556029 |
| Cycletest Plus DNA Reagent Kit | Trypsin solution, trypsin inhibitor/ribonuclease A solution, propidium iodide solution |
40 Tests | 340242 |
| DNA QC Particles | Chicken erythrocyte nuclei (CEN), Calf thymocyte nuclei (CTN), Instrument QC beads; Propidium iodide solution | 25 Tests | 349523 |
Anti-BrdU antibody
| 품명 | 용량 | 품번 |
| BrdU – Alexa Fluor® 488 | 100 Tests | 558599 |
| BrdU – Alexa Fluor® 555 | 100 Tests | 560210 |
| BrdU – Alexa Fluor® 647 | 100 Tests | 560209 |
| BrdU – BV510 | 50 Tests | 563445 |
| BrdU – FITC with DNase | 50 Tests | 340649 |
| BrdU – FITC | 100 Tests | 347583 |
| BrdU – PerCP | 50 Tests | 560808 |
| BrdU – PerCP-Cy™5.5 | 50 Tests | 560809 |
| BrdU – Purified | 0.1 mg | 555627 |
| BrdU – Purified | 100 Tests | 347580 |
| BrdU – V450 | 50 µg | 560810 |
Related product
| 품명 | 용량 | 품번 |
| 7-AAD | 2.0 ml | 559925 |
| BD Cytofix/Cytoperm™ Fixation/Permeablization Kit | 250 Tests | 554714 |
| Bromodeoxyuridine (BrdU) | 25 mg | 550891 |
| Perm/Wash Buffer I | 125 ml | 557885 |
| Permeabilization Buffer | 10 ml | 561651 |
| Stain Buffer (FBS) | 500 ml | 554656 |
| PI/RNase Staining Buffer | 100 ml | 550825 |
