줄기세포 연구의 모든 것, 3D Culture
3D culture란?
생체 내와 같은 입체적인 환경을 조성해 주어 세포 본래의 특성이 재현되도록 하는 세포 배양법
3D culture의 필요성
2D vs. 3D Culture
2D Cell Culture | 3D Cell Culture | |
Advantages | • Well established for primary assessment
• Economical • Easy and convenient for the analysis set up • High throughput capacity: feasible |
• Substrates mimic the natural extracellular matrix
• Mimic the in vivo microenvironment, as well as cell-cell and cell-matrix interaction • Enable the assessment of tissue penetration ability and bystander killing efficiency of drugs, as well as the drug resistance of cells. • Low design-in vitro-in vivo-redesign requirement: reduce overall cost for the pre-clinical in vivo test |
Disadvantages | • Lack cell-cell & cell-matrix interaction information
• Unable to mimic in vivo microenvironment • Homogenous drug distribution • Unable to assess the tissue penetration ability and bystander killing efficiency of drugs or the drug resistance of cells |
• Relatively low throughput capacity
• More expensive and laborious for the establishment of culture models and set-up of analyzing experiments |
3D culture plate 제품 소개
Brand | Description | Part Number | |
3D culture |
Reprocell | Alvetex®Scaffold 12 Well Plate×2 | AVP002-2 |
Alvetex®Scaffold 24 Well Plate×2 | AVP006-2 | ||
Alvetex®Scaffold 96 Well Plate×2 | AVP009-2 | ||
WAKO | PrimeSurface plate 24F | 627-01419 | |
PrimeSurface 96U White plate | 621-01439 | ||
PrimeSurface 96V plate | 629-01099 | ||
PrimeSurface 96U plate | 635-21039 | ||
PrimeSurface 384U plate | 628-01449 | ||
PrimeSurface 384U White plate | 625-01459 | ||
PrimeSurface 35mm dish | 009-77000MS-9035XZ | ||
PrimeSurface 60mm dish | 009-77000MS-9060XZ | ||
PrimeSurface 90mm dish | 009-77000MS-9090XZ |