cDNA and ORF Libraries

cDNA and ORFs

브랜드	Dharmacon™

제품특징

CCSB Human ORFeome Libraries

The Gateway adapted human Entry ORF libraries are ideal for easily moving entire families of ORFs into your choice of protein expression systems.

Precision LentiORFs

Precision LentiORFs are expression-ready open reading frames (ORFs) that have been cloned into a lentiviral expression vector with a GFP reporter.

ORFeome Collaboration Collection

ORFeome Collaboration clones are sequence-verified, human open reading frames (ORFs) subcloned into Gateway® recombinational entry vectors.

Mammalian Gene Collection (MGC) Clones

The mouse and human MGC libraries contain popular gene families in expression-ready and other vector backbones.

CCSB-Broad Lentiviral Expression Library

A genome-scale library of human ORFS in a lentiviral vector, stop codons have been removed and clones contain a C-terminal V5 tag.

Human ORFeome V8.1 Library

This fully-sequenced collection of Gateway-adapted ORFs represents the latest version of the CCSB Human ORFeome library from Dana-Farber Cancer Institute.

Cherry-pick Clone Libraries

Choose from our predesigned product lines of shRNA and over-expression reagents to build your own custom library.

제품스펙

Predefined Gene Expression Libraries

	HUMAN (number of genes)
Predefined Gene Expression Libraries	Fully-sequenced cDNA sets	Expression-ready cDNA sets	CCSB Human ORFeome	Precision LentiORF	hORFeome v8.1	CCSB-Broad Lentiviral Expression library
Genome	16,790	6,692	12,794	11,822	11,381	13,248
Apoptosis				315	297	285
Cell Cycle Regulation		291	498	111	101	100
Cytokine Receptors	220	67	130	76	63	62
Deubiquitinating Enzymes				56	57	56
DNA Damage Response				158	144	141
Drug Targets				3,256	2,934	2,870
Druggable Genome				4,907	4,490	4,388
Epigenetics				289	252	245
GPCRs	380	105	173	214	213	208
Growth Factor	175	60	90
Ion Channels	263	75	133	198	191	184
Kinase	714	289	428
Membrane Trafficking				96	90	88
Nuclear Receptors	47	20	32	30	30	30
Oxygen Binding			26
Peptidase	514	186	314
phosphatases	157	64	104	164	167	163
Plasma Membrane	2,207	779	1,240
Proteases				319	290	283
Protein Kinase	480	191	271	428	380	375
Serine Proteases				98	87	84
Transcription Factors	756	282	415	940	792	771
Transporter	1,056	373	635
Tyrosine Kinases				38	37	35
Ubiquitin	143	30	96
Ubiquitin Conjugation				352	336	326

주요사항

CCSB Human ORFeome highlights

• Genome-scale coverage - over 12,000 human genes available as ORF clones

• Gateway entry vectors facilitate convenient transfer of ORFs into compatible prokaryotic, mammalian, viral, or insect expression systems

• Native stop codons have been removed to allow for expression of tagged fusion proteins

Precision LentiORF Library highlights

• Genome-scale library of human open reading frames

• Lentiviral ORF constructs require no subcloning for mammalian expression

• Fully sequenced coding sequences

• GFP reporter is transported to the nucleus for easy visualization of expression

• Clones are available with or without native stop codons

• Available as ready-to-transduce viral particles, glycerol stocks, or LentiORF Starter Kits

ORFeome Collaboration Collection highlights

• Genome-scale ORF collection

• Easily transferable into any Gateway-adapted expression vector

• Available with and without stop codons

CCSB-Broad Lentiviral Expression Library highlights

• Genome-scale expression library of Human open reading frames

• CMV promoter drives ORF expression

• Blasticidin resistance allows for selection of transduced cells

• C-terminal V5 tag for additional utility

• Native stop codons have been removed

Human ORFeome V8.1 Library highlights