Products

주요제품

Dharmacon™  >  Small Interfering RNA (siRNA)

Small Interfering RNA (siRNA)

siGENOME siRNA, ON-TARGETplus siRNA, Lincode siRNA, Accell siRNA, Custom siRNA

제품용도 유전자 발현억제 (knockdown)
브랜드 Dharmacon™
개요 Dharmacon™ siRNA
제품특징

Dharmacon siRNA products are the result of scientific innovation in siRNA design and novel modification strategies to optimize potency, specificity and delivery.

 

>ON-TARGETplus siRNA

Enjoy optimal specificity from the only siRNA with a patented modification pattern to reduce off-targets caused by either the sense or the antisense strand.

>Accell siRNA

Specially modified for delivery into difficult-to-transfect cells without a transfection reagent, Accell enables non-viral RNAi in neuronal, immune, and stem cells.

>siGENOME siRNA

Highly potent siRNA for all human, mouse, and rat genes. siGENOME has provided the longest standing siRNA silencing guarantee on the market.

>Lincode siRNA

siRNAs for efficient knockdown of long, noncoding RNA (lncRNA) genes with modifications to ensure specificity.

>Custom siRNA Synthesis

No pre-designed product to fit your needs? Use our online design tools and extensive synthesis options to create a custom siRNA specific for your application.

제품스펙

Dharmacon offers four complete pre-designed product lines across human, mouse and rat genomes.

Use the table below to assist you in determining the right siRNA product line for your needs.

Cost-effective,
efficient silencing
High specificity for
reduced off-targets
+ efficient silencing
Highly specific
knockdown of long
noncoding RNA (lncRNA)
Target silencing in
difficult-to-transfect cells
siGENOME siRNA ON‑TARGETplus siRNA Lincode siRNA Accell siRNA
Pre-designed for
Human, Mouse and Rat
protein-coding genes
Pre-designed for Human and Mouse
long noncoding RNA (lncRNA)
Recommended for transfectable
mammalian cells in culture
Recommended for neuronal,
suspension, primary and
other difficult-to-transfect cells
Recommended transfection reagent DharmaFECT transfection
reagent
DharmaFECT transfection
reagent
DharmaFECT transfection
reagent
None required
Available as SMARTpool reagent
Available as four individual siRNAs
Guaranteed knockdown by
SMARTpool and 3 of 4 siRNAs
Sense strand is modified to prevent
interaction with RISC and favor
antisense strand uptake
Selective application
when thermodynamic
analysis indicates it
necessary for favorable
antisense RISC loading
Antisense strand seed region
is modified to destabilize off-target
activity and enhance target specificity
Modifications to facilitate cellular
uptake without separate transfection
reagents
Stabilizing modifications to prevent
nuclease-mediated degradation
Sequence information
provided with purchase
주요사항

>siRNA design

Our scientists were the first to establish siRNA design rules for high potency silencing and continue to drive innovation in this area. The SMARTselection algorithm selects highly functional and specific siRNAs for all of our predesigned products, enabling us to offer a functionality guarantee for all three of our major product lines: ON-TARGETplus, siGENOME, and Accell reagents.

>SMARTpool technology

Pooling of siRNAs better mimics the natural RNAi pathway, and when combined with SMARTselection design, provides advantages for both potency and specificity.

-Targeting four mRNA regions at once improves the likelihood of effective silencing and reduces false negatives due to inaccessible binding sites

-Lower relative concentration of each siRNA reduces sequence-specific off-targeting

-All Dharmacon predesigned siRNA products are all available as SMARTpool reagents or up to 4 individual siRNAs

>Reducing off-targets for better specificity

The primary source of siRNA-mediated off targets is the seed region (nucleotides 2-7) which uses the microRNA pathway to induce non-specific gene silencing via interactions within the 3' UTR. Seed region filters and seed frequency analysis are incorporated in the SMARTselection siRNA design strategy to reduce microRNA-like off-target activity. Dharmacon siRNA products provide the most comprehensive bioinformatic strategies to mitigate off-targeting.

-Conserved microRNA seed regions are filtered out of siRNA designs

-Frequency analysis of siRNA seed complements (how often this occurs in the 3' UTR transcriptome) are performed and preference is given to siRNA designs with low-frequency seeds