Small Interfering RNA (siRNA)
siGENOME siRNA, ON-TARGETplus siRNA, Lincode siRNA, Accell siRNA, Custom siRNA
Dharmacon siRNA products are the result of scientific innovation in siRNA design and novel modification strategies to optimize potency, specificity and delivery.
Enjoy optimal specificity from the only siRNA with a patented modification pattern to reduce off-targets caused by either the sense or the antisense strand.
Specially modified for delivery into difficult-to-transfect cells without a transfection reagent, Accell enables non-viral RNAi in neuronal, immune, and stem cells.
Highly potent siRNA for all human, mouse, and rat genes. siGENOME has provided the longest standing siRNA silencing guarantee on the market.
siRNAs for efficient knockdown of long, noncoding RNA (lncRNA) genes with modifications to ensure specificity.
>Custom siRNA Synthesis
No pre-designed product to fit your needs? Use our online design tools and extensive synthesis options to create a custom siRNA specific for your application.
Dharmacon offers four complete pre-designed product lines across human, mouse and rat genomes.
Use the table below to assist you in determining the right siRNA product line for your needs.
|High specificity for
+ efficient silencing
knockdown of long
noncoding RNA (lncRNA)
|Target silencing in
|siGENOME siRNA||ON‑TARGETplus siRNA||Lincode siRNA||Accell siRNA|
Human, Mouse and Rat
|Pre-designed for Human and Mouse
long noncoding RNA (lncRNA)
|Recommended for transfectable
mammalian cells in culture
|Recommended for neuronal,
suspension, primary and
other difficult-to-transfect cells
|Recommended transfection reagent||DharmaFECT transfection
|Available as SMARTpool reagent||●||●||●||●|
|Available as four individual siRNAs||●||●||●||●|
|Guaranteed knockdown by
SMARTpool and 3 of 4 siRNAs
|Sense strand is modified to prevent
interaction with RISC and favor
antisense strand uptake
analysis indicates it
necessary for favorable
antisense RISC loading
|Antisense strand seed region
is modified to destabilize off-target
activity and enhance target specificity
|Modifications to facilitate cellular
uptake without separate transfection
|Stabilizing modifications to prevent
provided with purchase
Our scientists were the first to establish siRNA design rules for high potency silencing and continue to drive innovation in this area. The SMARTselection algorithm selects highly functional and specific siRNAs for all of our predesigned products, enabling us to offer a functionality guarantee for all three of our major product lines: ON-TARGETplus, siGENOME, and Accell reagents.
Pooling of siRNAs better mimics the natural RNAi pathway, and when combined with SMARTselection design, provides advantages for both potency and specificity.
-Targeting four mRNA regions at once improves the likelihood of effective silencing and reduces false negatives due to inaccessible binding sites
-Lower relative concentration of each siRNA reduces sequence-specific off-targeting
-All Dharmacon predesigned siRNA products are all available as SMARTpool reagents or up to 4 individual siRNAs
>Reducing off-targets for better specificity
The primary source of siRNA-mediated off targets is the seed region (nucleotides 2-7) which uses the microRNA pathway to induce non-specific gene silencing via interactions within the 3' UTR. Seed region filters and seed frequency analysis are incorporated in the SMARTselection siRNA design strategy to reduce microRNA-like off-target activity. Dharmacon siRNA products provide the most comprehensive bioinformatic strategies to mitigate off-targeting.
-Conserved microRNA seed regions are filtered out of siRNA designs
-Frequency analysis of siRNA seed complements (how often this occurs in the 3' UTR transcriptome) are performed and preference is given to siRNA designs with low-frequency seeds