
Amersham™ ECL
최고의 품질과 최고의 가성비를 가진 Amersham ECL 제품
브랜드 | Cytiva |
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제품특징
Western Blotting Detection
Amersham ECL Select (Low picogram, <5pg)
Amersham ECL range 중 가장 감도가 높은 시약입니다. 단백질 발현의 작은 변화도 검출 가능하며, Low picograms(<5pg) 수준의 단백질까지 검출할 수 있습니다.
Amersham ECL Prime (Mid picogram, 10-100pg)
고감도 시약으로서, 시간에 따른 단백질 수준의 변화를 정확히 검출할 수 있도록 24시간 이상의 signal duration을 가집니다.
Amersham ECL (Mid picogram, 20-100pg)
Standard ECL 시약으로서, Mid picograms(20-100pg)수준의 단백질 검출이 가능합니다.
Amersham ECL Start (nanogram 단위)
가장 보편적으로 많이 사용하는 가성비 좋은 ECL 시약으로서, nanogram 수준의 단백질 검출이 가능합니다.
제품스펙
Amersham™ ECL™ Wester Blotting Detection Reagents
품명 | 규격 | 품번 |
Amersham™ ECL Select™ | 100 ml | RPN2235 |
Amersham™ ECL™ Prime | 100 ml | RPN2232 |
Amersham™ ECL™ | 500 ml | RPN2106 |
250 ml | RPN2209 | |
125 ml | RPN2109 | |
Amersham™ ECL™ start | 400 ml | RPN3244 |
200 ml | RPN3243 |
관련 제품
구분 | 품명 | 규격 | 품번 |
Membrane | Amersham™ Hybond™ P PVDF 0.2 μm | 260 mm x 4 m | 10600021 |
Amersham™ Hybond™ P PVDF 0.45 μm | 300 mm x 4 m | 10600023 | |
Amersham™ Protran™ NC 0.2 μm | 300 mm x 4 m | 10600001 | |
Amersham™ Protran™ NC 0.4 μm | 300 mm x 4 m | 10600002 | |
Marker | Amersham™ ECL DualVue™ WB Markers | 25 gel loadings | RPN810 |
Amersham™ ECL™ Full-Range Rainbow Markers | 250 μl | RPN800E | |
HRP conjugated secondary antibodies | Amersham™ ECL™ Mouse IgG HRP-Linked Whole Antibody | 1 ml | NA931-1ML |
Amersham™ ECL™ Rabbit IgG HRP-Linked Whole Antibody | 1 ml | NA934-1ML |
주요사항
Benchmarking data
Amersham ECL Select
Figure 8 shows a side-by-side comparison of Amersham ECL Select with other high-sensitive chemiluminescence detection reagents.Samples: Two-fold dilution series of HeLa cell lysate starting at 2.5 μg Primary antibody dilution: 1:10 000 Secondary antibody dilution: 1:100 000 Detection: Amersham Imager 600, 3 min exposure |
Fig 8. Amersham ECL Select shows brighter bands, lower background, |
Amersham ECL Prime
Figure 6 shows a side-by-side comparison of Amersham ECL Prime with other high-sensitive chemiluminescence detection reagents.Samples: Two-fold dilution series of HeLa cell lysate starting at 10 μg Primary antibody dilution: 1:5000 Secondary antibody dilution: 1:30 000 Detection: Amersham Imager 600, 75 s exposure ![]() reagents in Western blotting detection of ERK 1/2. The Millipore reagents were slightly less sensitivity and also reached saturation at the highest concentrations (10 to 5 μg), decreasing the dynamic range of these reagents. |
Figure 7 shows Amersham ECL Prime in comparison with Clarity™ Western ECL Substrate from Bio-Rad.Samples: Two-fold dilution series of HeLa cell lysate starting at 2.5 μg Primary antibody dilution: 1:3000 Secondary antibody dilution: 1:30 000 Detection: Amersham Imager 600, 3 min exposure ![]() compared with Clarity Western ECL Substrate in Western blotting detection of ERK 1/2. |
Amersham ECL
Figure 5 shows a side-by-side comparison of Amersham ECL reagent with other chemiluminescence detection reagents in the same sensitivity range.Samples: Two-fold dilution series of HeLa cell lysate starting at 5 μg Primary antibody dilution: 1:1000 Secondary antibody dilution: 1:10 000 Detection: Amersham Imager 600, 1 min exposure |
Fig 5. Amersham ECL reagent exhibits similar sensitivity as the competitor |
Amersham ECL Start
An assessment of signal duration for Amersham ECL start Western blotting detection reagent over time indicated that;(A). The signal duration allows for the sustained detection of high to medium protein levels for up to 3 h; | ![]()
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