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Cytiva  >  Amersham™ ECL

Amersham™ ECL

최고의 품질과 최고의 가성비를 가진 Amersham ECL 제품

브랜드 Cytiva
제품특징

Amersham™ ECL 샘플신청 바로가기

 

Western Blotting Detection

Amersham ECL Select (Low picogram, <5pg)
Amersham ECL range 중 가장 감도가 높은 시약입니다. 단백질 발현의 작은 변화도 검출 가능하며, Low picograms(<5pg) 수준의 단백질까지 검출할 수 있습니다.

 

Amersham ECL Prime (Mid picogram, 10-100pg)
고감도 시약으로서, 시간에 따른 단백질 수준의 변화를 정확히 검출할 수 있도록 24시간 이상의 signal duration을 가집니다.

 

Amersham ECL (Mid picogram, 20-100pg)
Standard ECL 시약으로서, Mid picograms(20-100pg)수준의 단백질 검출이 가능합니다.

 

Amersham ECL Start (nanogram 단위)

가장 보편적으로 많이 사용하는 가성비 좋은 ECL 시약으로서, nanogram 수준의 단백질 검출이 가능합니다.

제품스펙

Amersham™ ECL™ Wester Blotting Detection Reagents

품명 규격 품번
Amersham™ ECL Select™ 100 ml RPN2235
Amersham™ ECL™ Prime 100 ml RPN2232
Amersham™ ECL™ 500 ml RPN2106
250 ml RPN2209
125 ml RPN2109
Amersham™ ECL™ start 400 ml RPN3244
200 ml RPN3243

관련 제품

구분 품명 규격 품번
Membrane Amersham™ Hybond™ P PVDF 0.2 μm 260 mm x 4 m 10600021
Amersham™ Hybond™ P PVDF 0.45 μm 300 mm x 4 m 10600023
Amersham™ Protran™ NC 0.2 μm 300 mm x 4 m 10600001
Amersham™ Protran™ NC 0.4 μm 300 mm x 4 m 10600002
Marker Amersham™ ECL DualVue™ WB Markers 25 gel loadings RPN810
Amersham™ ECL™ Full-Range Rainbow Markers 250 μl RPN800E
HRP conjugated secondary antibodies Amersham™ ECL™ Mouse IgG HRP-Linked Whole Antibody 1 ml NA931-1ML
Amersham™ ECL™ Rabbit IgG HRP-Linked Whole Antibody 1 ml NA934-1ML
주요사항

Benchmarking data

 

Amersham ECL Select

Figure 8 shows a side-by-side comparison of
Amersham ECL Select with other high-sensitive
chemiluminescence detection reagents.Samples: Two-fold dilution series of HeLa cell lysate starting at 2.5 μg
Primary antibody dilution: 1:10 000
Secondary antibody dilution: 1:100 000
Detection: Amersham Imager 600, 3 min exposure		  

Fig 8. Amersham ECL Select shows brighter bands, lower background,
and higher limit of detection compared with competitor reagents in
Western blotting detection of ERK 1/2.

Amersham ECL Prime

Figure 6 shows a side-by-side comparison of
Amersham ECL Prime with other high-sensitive
chemiluminescence detection reagents.Samples: Two-fold dilution series of HeLa cell lysate starting at 10 μg
Primary antibody dilution: 1:5000
Secondary antibody dilution: 1:30 000
Detection: Amersham Imager 600, 75 s exposureFig 6. Amersham ECL Prime exhibits similar sensitivity as the competitor
reagents in Western blotting detection of ERK 1/2. The Millipore reagents
were slightly less sensitivity and also reached saturation at the highest
concentrations (10 to 5 μg), decreasing the dynamic range of these reagents.		 Figure 7 shows Amersham ECL Prime in comparison
with Clarity™ Western ECL Substrate from Bio-Rad.Samples: Two-fold dilution series of HeLa cell lysate starting at 2.5 μg
Primary antibody dilution: 1:3000
Secondary antibody dilution: 1:30 000
Detection: Amersham Imager 600, 3 min exposureFig 7. Amersham ECL Prime exhibits higher sensitivity and signal intensity
compared with Clarity Western ECL Substrate in Western blotting
detection of ERK 1/2.

Amersham ECL

Figure 5 shows a side-by-side comparison of
Amersham ECL reagent with other chemiluminescence
detection reagents in the same sensitivity range.Samples: Two-fold dilution series of HeLa cell lysate starting at 5 μg
Primary antibody dilution: 1:1000
Secondary antibody dilution: 1:10 000
Detection: Amersham Imager 600, 1 min exposure		  

Fig 5. Amersham ECL reagent exhibits similar sensitivity as the competitor
reagents in Western blotting detection of ERK 1/2.

Amersham ECL Start

An assessment of signal duration for Amersham ECL start Western blotting detection reagent over time indicated that;(A). The signal duration allows for the sustained detection of high to medium protein levels for up to 3 h;