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주요제품

Dharmacon™  >  siRNA Screening Libraries

siRNA Screening Libraries

RNAi Screening Libraries

제품용도 유전자 발현억제 (knockdown)
브랜드 Dharmacon™
개요 siRNA Screening Libraries
제품특징

Human siRNA Libraries

Find the predefined gene family, including Druggable genes or Whole Genome, that’s right for your discovery efforts. Human siRNA libraries are available in SMARTpool or individual siRNA format for ON-TARGETplus, siGENOME and Accell technologies. Learn more about each product line to make the right choice for your experimental needs. We support more strategies for successful screening and more screening expertise than any other provider.

Mouse siRNA Libraries

A wide selection of predefined siRNA libraries, including the most up-to-date Whole Mouse genome collection available. Mouse siRNA libraries are available in SMARTpool or individual siRNA format for ON-TARGETplus, siGENOME and Accell technologies. Learn more about each product line to make the right choice for your experimental needs. We support more strategies for successful screening and more screening expertise than any other provider.

RTF siRNA Libraries

Reverse Transfection Format (RTF) siRNA libraries are provided as multiple single-use plate sets – just rehydrate and add cells. This unique pre-plated format reduces hands-on time for faster screening results.

RTF SMARTpool siRNA Libraries are ideal for the lab that wants to screen with siRNA, but doesn't have access to high-throughput automation. SMARTpool siRNA and validated control reagents are pre-dispensed at assay-ready amounts in replicate 96-well cell culture plates. No special handling is required prior to use. Simply resuspend the siRNA and add your cells.

Lincode siRNA Libraries

Unbiased RNAi screens have become an important tool for elucidation of novel biological pathways and disease progression. The assessment of potential roles for microRNA and lncRNA, in addition to protein-coding genes, can provide a more complete picture of the genes involved in the phenotype of interest.

Human and Mouse Lincode siRNA screening libraries are available as SMARTpool or a Set of 4 individual siRNAs to support efforts to decipher lncRNA function.

Cherry-pick Libraries

Fast and easy online configuration and ordering of plated siRNA & microRNA reagents targeting your genes of interest.

Zoonome Custom siRNA Libraries

Enzymatically generated custom siRNA libraries for screening in non-human or mouse model organisms.

 

 

 

제품스펙

Predefined siRNA Libraries

HUMAN
(number of genes)
MOUSE
(number of genes)
Predefined siRNA Libraries siGENOME ON-TARGETplus Accell siGENOME ON-TARGETplus Accell
Ion Channels 349 349 347 340 340 331
Phosphatases 256 254 257 273 272 243
Protein Kinases 712 709 714 715 714 652
Ubiquitin Conjugation Subset #1 88 87 89 80 80 82
Ubiquitin Conjugation Subset #2 113 115 1185 104 104 109
Ubiquitin Conjugation Subset #3 379 386 396 339 339 351
Deubiquitinating Enzymes 98 98 103 68 65
Drug Targets 4,795 4,786 4,799 7,100 7,096
Druggable Genome 7,582 7,567 7,587 10,006 9,998
G Protein-Coupled Receptors(GPCR) 390 390 390 515 513
Proteases 480 477 480 540 540 454
Apoptosis 446 558 289 289
Cell Cycle Regulation 169 131 105 128
Nuclear Receptors 52 52 52 46 46
Tyrosine Kinases 85 88 85 85
Genome 18,164 18,104 19,098
Membrane Trafficking 140 140 113 112
Cytokine Receptors 116 116 158 158
DNA Damage Response 240 240
Transcription Factors 1,529 1,440 1,436
Epigenetics 845 835 730 730
Long noncoding RNA(Lincode siRNA) 3,461 1,997

 

주요사항

RTF siRNA Libraries Benefits

• Each library is supplied as six replicate plate sets containing 6.25 nmol of siRNA per well; sufficient to run two screens in triplicate (additional plate sets available)

• RTF Optimization Plates are available to optimize transfection, controls, and cell density for your assay prior to beginning your screen

• RTF libraries are available in clear-bottom plates with clear, black, or white-walled wells to support assays involving fluorescent or luminescent detection methods

 

Lincode siRNA Libraries Benefits

• Patented dual-strand modification pattern on all siRNAs to reduce off-targets

• Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake

• Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity

• Available as SMARTpool siRNA reagents or a Set of 4 siRNAs in 96-well or 384-well plates