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주요제품

Dharmacon™  >  cDNA and ORF Libraries

cDNA and ORF Libraries

cDNA and ORFs

제품용도 유전자 과발현 (Over Expression)
브랜드 Dharmacon™
개요 Pre-defined gene family collections are available for many of our cDNA and ORF products.
제품특징

CCSB Human ORFeome Libraries

The Gateway adapted human Entry ORF libraries are ideal for easily moving entire families of ORFs into your choice of protein expression systems.

Precision LentiORFs

Precision LentiORFs are expression-ready open reading frames (ORFs) that have been cloned into a lentiviral expression vector with a GFP reporter.

ORFeome Collaboration Collection

ORFeome Collaboration clones are sequence-verified, human open reading frames (ORFs) subcloned into Gateway® recombinational entry vectors.

Mammalian Gene Collection (MGC) Clones

The mouse and human MGC libraries contain popular gene families in expression-ready and other vector backbones.

CCSB-Broad Lentiviral Expression Library

A genome-scale library of human ORFS in a lentiviral vector, stop codons have been removed and clones contain a C-terminal V5 tag.

Human ORFeome V8.1 Library

This fully-sequenced collection of Gateway-adapted ORFs represents the latest version of the CCSB Human ORFeome library from Dana-Farber Cancer Institute.

Cherry-pick Clone Libraries

Choose from our predesigned product lines of shRNA and over-expression reagents to build your own custom library.

 

 

제품스펙

Predefined Gene Expression Libraries

HUMAN
(number of genes)
Predefined Gene
Expression Libraries
Fully-sequenced
cDNA sets
Expression-ready
cDNA sets
CCSB Human
ORFeome
Precision
LentiORF
hORFeome
v8.1
CCSB-Broad Lentiviral
Expression library
Genome 16,790 6,692 12,794 11,822 11,381 13,248
Apoptosis 315 297 285
Cell Cycle Regulation 291 498 111 101 100
Cytokine Receptors 220 67 130 76 63 62
Deubiquitinating Enzymes 56 57 56
DNA Damage Response 158 144 141
Drug Targets 3,256 2,934 2,870
Druggable Genome 4,907 4,490 4,388
Epigenetics 289 252 245
GPCRs 380 105 173 214 213 208
Growth Factor 175 60 90
Ion Channels 263 75 133 198 191 184
Kinase 714 289 428
Membrane Trafficking 96 90 88
Nuclear Receptors 47 20 32 30 30 30
Oxygen Binding 26
Peptidase 514 186 314
phosphatases 157 64 104 164 167 163
Plasma Membrane 2,207 779 1,240
Proteases 319 290 283
Protein Kinase 480 191 271 428 380 375
Serine Proteases 98 87 84
Transcription Factors 756 282 415 940 792 771
Transporter 1,056 373 635
Tyrosine Kinases 38 37 35
Ubiquitin 143 30 96
Ubiquitin Conjugation 352 336 326
주요사항

CCSB Human ORFeome highlights

• Genome-scale coverage - over 12,000 human genes available as ORF clones

• Gateway entry vectors facilitate convenient transfer of ORFs into compatible prokaryotic, mammalian, viral, or insect expression systems

• Native stop codons have been removed to allow for expression of tagged fusion proteins

 

Precision LentiORF Library highlights

• Genome-scale library of human open reading frames

• Lentiviral ORF constructs require no subcloning for mammalian expression

• Fully sequenced coding sequences

• GFP reporter is transported to the nucleus for easy visualization of expression

• Clones are available with or without native stop codons

• Available as ready-to-transduce viral particles, glycerol stocks, or LentiORF Starter Kits

 

ORFeome Collaboration Collection highlights

• Genome-scale ORF collection

• Easily transferable into any Gateway-adapted expression vector

• Available with and without stop codons

 

CCSB-Broad Lentiviral Expression Library highlights

• Genome-scale expression library of Human open reading frames

• CMV promoter drives ORF expression

• Blasticidin resistance allows for selection of transduced cells

• C-terminal V5 tag for additional utility

• Native stop codons have been removed

 

Human ORFeome V8.1 Library highlights

• Genome-scale library of human open reading frames

• Gateway –adapted for efficient transfer to compatible expression vectors

• Native stop codons have been removed