줄기세포 연구의 모든 것, 3D Culture


3D culture?

생체 내와 같은 입체적인 환경을 조성해 주어 세포 본래의 특성이 재현되도록 하는 세포 배양법


3D culture의 필요성


2D vs. 3D Culture

2D Cell Culture 3D Cell Culture
Advantages • Well established for primary assessment

• Economical

• Easy and convenient for the analysis set up

• High throughput capacity: feasible

• Substrates mimic the natural extracellular matrix

Mimic the in vivo microenvironment, as well as cell-cell and cell-matrix interaction

• Enable the assessment of tissue penetration ability and bystander killing efficiency of drugs, as well as the drug resistance of cells.

• Low design-in vitro-in vivo-redesign requirement: reduce overall cost for the pre-clinical in vivo test

Disadvantages • Lack cell-cell & cell-matrix interaction information

• Unable to mimic in vivo microenvironment

• Homogenous drug distribution

• Unable to assess the tissue penetration ability and bystander killing efficiency of drugs or the drug resistance of cells

• Relatively low throughput capacity

• More expensive and laborious for the establishment of culture models and set-up of analyzing experiments


3D culture plate 제품 소개

Brand Description Part Number






3D culture

Reprocell Alvetex®Scaffold 12 Well Plate×2 AVP002-2
Alvetex®Scaffold 24 Well Plate×2 AVP006-2
Alvetex®Scaffold 96 Well Plate×2 AVP009-2
WAKO PrimeSurface plate 24F 627-01419
PrimeSurface 96U White plate 621-01439
PrimeSurface 96V plate 629-01099
PrimeSurface 96U plate 635-21039
PrimeSurface 384U plate 628-01449
PrimeSurface 384U White plate 625-01459
PrimeSurface 35mm dish 009-77000MS-9035XZ
PrimeSurface 60mm dish 009-77000MS-9060XZ
PrimeSurface 90mm dish 009-77000MS-9090XZ